第一种方式,直接在Aspx页面GridView模板列中.这种的缺点是到第二页分页时又重新开始了.

            <asp:TemplateField HeaderText="序号" InsertVisible="False">

              <ItemStyle HorizontalAlign="Center" />
              <HeaderStyle HorizontalAlign="Center" Width="5%" />
             <ItemTemplate>
              <%#Container.DataItemIndex+1%>
            </ItemTemplate>
            </asp:TemplateField>

第二种方式分页时进行了计算,这样会累计向下加.

            <asp:TemplateField HeaderText="序号" InsertVisible="False">

              <ItemStyle HorizontalAlign="Center" />
              <HeaderStyle HorizontalAlign="Center" Width="5%" />
             <ItemTemplate>
                 <asp:Label ID="Label2" runat="server" Text='<%# this.MyListGridView.PageIndex * this.MyListGridView.PageSize + this.MyListGridView.Rows.Count + 1%>'/>
            </ItemTemplate>
            </asp:TemplateField>

还有一种方式放在cs代码中,和第二种相似.

    <asp:BoundField HeaderText="序号" >

              <ItemStyle HorizontalAlign="Center" />
              <HeaderStyle HorizontalAlign="Center" Width="5%" />
          </asp:BoundField>

        protected void myGridView_RowDataBound(object sender, GridViewRowEventArgs e)
        {
            if (e.Row.RowIndex != -1)
            {
                int indexID = this.myGridView.PageIndex * this.myGridView.PageSize + e.Row.RowIndex + 1;
                 e.Row.Cells[0].Text = indexID.ToString();
             }
         }

http://www.cnblogs.com/lin614/archive/2008/07/24/1250839.html